| 蚯蚓MT基因的克隆及其在大肠杆菌中的表达 |
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| 引用本文： | 刘兰芳,李继刚,米国桥. 蚯蚓MT基因的克隆及其在大肠杆菌中的表达[J]. 河北农业大学学报, 2010, 33(1) |
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| 作者姓名： | 刘兰芳 李继刚 米国桥 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002;河北大学,生命科学学院,河北,保定,071002;河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省教育厅项目(2007412) |
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| 摘 要： | 为了研究蚯蚓金属硫蛋白的生物学功能,采用RT-PCR方法从镉诱导后的赤子爱胜蚓(Eisenia foetida)中克隆到蚯蚓金属硫蛋白基因cDNA。将其克隆入原核表达载体pET-DsbA中,然后转化至大肠杆菌表达受体菌BL21(DE3)中进行表达。产物经SDS-PAGE进行分析,可见约32.4 kD的融合蛋白。Zn2+抗性测定表明含pET-DsbA-efMT的重组菌较含空载体pET-DsbA的对照菌重金属抗性有所提高。
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| 关 键 词： | MT基因 克隆 原核表达 抗性 |
Cloning,expression of Eisenia foetida metallothionein gene in Escherichia coli |
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| LIU Lan-fang,LI Ji-gang,MI Guo-qiao. Cloning,expression of Eisenia foetida metallothionein gene in Escherichia coli[J]. Journal of Agricultural University of Hebei, 2010, 33(1) |
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| Authors: | LIU Lan-fang LI Ji-gang MI Guo-qiao |
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| Affiliation: | College of Life Sciences;Hebei University;Baoding 071002;China |
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| Abstract: | To study the function of Eisenia foetida metallothionein,the cDNA fragment encoding the Eisenia foetida metallothionein was amplified by RT-PCR from the Eisenia foetida induced by cadmium and cloned into prokaryotic expression vector pET-DsbA.After transformation of the recombinant plasmid into expression host Escherichia coli BL21(DE3),the expression product was analyzed by SDS-PAGE electrophoresis,and an approximately 32.4 kDa fusion protein was detected.Zinc ion resistance assay showed that the recombina... |
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| Keywords: | metallothionein gene clone prokaryotic expression resistance |
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