外源添加抑制剂对芦蒿吸收Cd和Pb的影响

以水生植物芦蒿为试验材料,通过人工培养液温室培养方法,研究了代谢抑制剂、P-型ATP酶抑制剂、蛋白合成抑制剂、离子通道抑制剂对芦蒿吸收Cd和Pb的影响。结果表明,代谢抑制剂（Na3N）和P-型ATP酶抑制剂（Na3VO4）对芦蒿Cd和Pb的吸收均有一定的抑制作用,说明芦蒿对Cd和Pb存在主动吸收。钙离子通道抑制剂LaCl3显著抑制芦蒿根部对Cd的吸收,而对Pb的吸收没有明显的抑制作用,说明芦蒿对Cd的吸收与钙离子通道有关;蛋白质合成抑制剂放线菌酮（CHD）对芦蒿Cd和Pb的吸收有显著的抑制作用,说明芦蒿对Cd和Pb的吸收与一些蛋白质或酶的诱导合成及其活性密切相关。

Effect of Additive Inhibitor on the Cd and Pb Uptake of Artemisia seleirgensis

Artemisia seleirgensis is a popular vegetable in Nanjing. It provides people with kinds of vitamines, celluloses and minerals. However, it is easily contaminated by heavy metal, which is harmful to people＇ s health. So it is very important to study on the mechanism of heavy metal absorption ofA. seleirgensis. To reduce heavy metal adsorption and develop pollution-free vegetables, the paper investigated the effect of the inhibitor of respiration, P-type ATPase inhibitor, ion channels inhibitor, protein synthesis inhibitor on Cd and Pb uptake in A.seleirgensis. The results showed both the respiration inhibitor NaN3 and P-type ATPase inhibitor Na3VO4 could inhibit Cd and Pb uptake, which showed properly initiative Cd and Pb uptake in the plant. Ca^2＋ ion channel inhibitor LaCl3 inhibited Cd uptake ofA .seleirgensis in root, while did not inhibit Pb uptake,which demonstrated Cd uptake of A.seleirgensis was related to Ca^2＋ ion channel, while Pb uptake was not. Cd and Pb uptake of A.seleirgensis were inhibited by the protein synthesis inhibitor cyeloheximide, showed that Cd and Ph uptake related to inducement synthesize and activity of protein and enzyme. The results provided the theoretical basis on heavy metal reduction ofA.seleirgensis, and it had practical significance to improve the health of people.