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农药废水对秀丽隐杆线虫毒性效应及其主要有毒组分
引用本文:王晓祎,;王大勇,;李爱民,;于红霞,;刘晓华.农药废水对秀丽隐杆线虫毒性效应及其主要有毒组分[J].农业环境保护,2009(7):1392-1396.
作者姓名:王晓祎  ;王大勇  ;李爱民  ;于红霞  ;刘晓华
作者单位:[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210093; [2]东南大学基础医学院发育与疾病相关基因教育部重点实验室,江苏南京210009
基金项目:江苏省科技计划项目(BS2007050);南京大学研究生科研创新基金资助项目(2007CL11);江苏省环境监测科研基金项目(0710);江苏省普通高校研究生科研创新计划资助项目(CX07B_170z)
摘    要:应用battery生物测试法,检测研究农药废水对秀丽隐杆线虫的生命周期、繁殖速率、生殖能力、头摆和身体弯曲频率等影响的生物毒性,及其引发生物毒性的主要有机污染物。结果表明,在已经达到国家废水排放标准GB 8978—1996]情况下,处理出水对受试动物仍然存在致毒效应;线虫的世代周期对进水的毒性最为敏感,产卵数量对出水的毒性最为敏感;进水毒性主要来自易受酸性调节影响的有机污染物,出水毒性主要来自易受碱性调节影响的有机污染物。结果表明,该项生物测试的毒性参数,可用于指示存在于低CODCr废水中的生物毒性;所用的毒性鉴别评价(TIE)方法,可用于鉴别废水中引发致毒效应的关键污染物。废水中悬浮颗粒污染物的生物毒性至关重要,尚未研究,有待继续。

关 键 词:农药废水  秀丽隐杆线虫  生物测试  毒性评价  主要致毒物

Toxicity of A Pesticide Wastewater on Caenorhabditis elegans
Institution:WANG Xiao-yi, WANG Da-yong, LI Ai-min, YU Hong-xia, LIU Xiao-hua ( 1.State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China; 2.Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Basic Medical College, Southeast University, Nanjing 210009, China)
Abstract:The toxicities of influent and effluent for a pesticide wastewater treatment system on the model animal, Caenorhabditis elegan, were measured for identification of the target organic pollutants with the approach of toxicity identification evaluation(TIE ). Although the effluent quality could met the limit as the list in Chinese Integrated Wastewater Discharge StandardGB 8978-1996], both the influent and effluent still revealed their toxicities on C. elegans proved in the battery bioassays with the parameters including life span, generation time, brood size, head thrashes and body bends. The generation time for C. elegans was sensitive to the influent and the hrood size of C. elegans was sensitive to the effluent. The major toxicants in the intluent were acidic organic compounds but in the effluent were alkalescent organic chemicals. The results indicated that the bioassay methods could be used to test the toxicity existed in the low CODCr wastewater and the approach of toxicity identification evaluation should be effective for discovering target toxicants.
Keywords:pesticide wastewater  C  elegans  bioassay  toxicity evaluation  major toxicants
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