| 长三角地区有机农药污染地下水的微生物群落分析 |
| |
| 引用本文： | 吴海燕,;徐红霞,;洪宜斌,;马宏瑞,;邱宇平,;张景飞,;吴吉春. 长三角地区有机农药污染地下水的微生物群落分析[J]. 农业环境保护, 2009, 0(9): 1898-1902 |
| |
| 作者姓名： | 吴海燕, 徐红霞, 洪宜斌, 马宏瑞, 邱宇平, 张景飞, 吴吉春 |
| |
| 作者单位： | [1]南京大学水科学系,污染控制与资源化研究国家重点实验室,江苏南京210093; [2]中国石化管道储运公司,江苏南京211100; [3]陕西科技大学资源与环境学院,陕西西安710021; [4]浙江工业大学生物与环境学院,浙江杭州310032 |
| |
| 基金项目： | 国家自然科学青年基金（40502024）;国家杰出青年基金（40725010）;污染控制与资源化研究国家重点实验室开放基金（PCR200406）;浙江工业大学环境工程“重中之重”学科开放基金（20080206） |
| |
| 摘 要： | 选择长江三角洲地区一处长期受到有机农药污染的浅层含水层，采集水样，采用不依赖于培养的16SrDNA序列分析方法，对该污染地下水中的微生物群落特征进行了分析和鉴定。结果表明，这种分析方法完全可行，该有机污染含水层具有较好的微生物多样性，且可能有一种未经鉴定的新菌种。
|
| 关 键 词： | 有机污染 地下水 微生物多样性 16S rDNA |
Analysis for the Microbial Communities of an Aquifer in Yangtze River Delta Polluted Long-term by Organic Compounds |
| |
| Affiliation: | WU Hai-yan, XU Hong-xia, HONG Yi-bin, MA Hong-rui, QIU Yu-ping, ZHANG Jing-fei, WU Ji-chun ( 1 .Department of Hydroscience, State Key Laboratory of Pollution Control and Resources Reuse, Nanjing University, Nanjing 210093, China; 2.SINOPEC Pipeline Storage and Transportation Company, Nanjing 211100, China; 3.College of Resource ＆ Environment, Shaanxi University of Science and Technology, Xi＇ an 710021, China; 4.College of Biology ＆ Environment, Zhejiang University of Technology, Hangzhou 310032, China) |
| |
| Abstract: | A shallow aquifer, located in Yangtze River Delta and polluted by organic substances for long time, was chosed to study the microbial diversity and the relations of genus and species, adopting the 16S rDNA sequencing method, a microbial molecular ecology technique that analyzed microbial community and without any cultivation. Experimental results showed, the 16S rDNA sequencing method was well successful. The shallow aquifer suffering from long-time organic contamination had a good microbial diversity, while the bacterium belonging to an unknown genus was probably discovered. The present study builds a practical method for the identification of microbial diversity in the polluted groundwater and provides scientifical basis, to some extent, for the further studies on the biological remediation technology of groundwater polluted by organic compouds. |
| |
| Keywords: | organic contamination groundwater microbial diversity 16S rDNA |
| 本文献已被 维普 等数据库收录！ |
|
