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| SCAR分子标记鉴别烟粉虱和温室白粉虱 | |
| 引用本文： | 褚栋,;张友军;丛斌;徐宝云;吴青君. SCAR分子标记鉴别烟粉虱和温室白粉虱[J]. 植物保护, 2004, 30(6): 27-30 |
| 作者姓名： | 褚栋, 张友军 丛斌 徐宝云 吴青君 |
| 作者单位： | 1. 沈阳农业大学植物保护学院,沈阳,110161;中国农业科学院蔬菜花卉研究所,北京,100081 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 沈阳农业大学植物保护学院,沈阳,110161 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 在简单重复序列PCR的基础上建立了烟粉虱和白粉虱两个物种DNA特异性片段的SCAR标记。验证表明,新建立的烟粉虱SCAR标记能特异性扩增不同地理种群的烟粉虱而不能扩增温室白粉虱;相反新建立的温室白粉虱SCAR标记则能特异性地扩增不同地理种群的温室白粉虱而不能扩增烟粉虱种群。两种SCAR标记能够快速、准确地鉴定烟粉虱和温室白粉虱。 |
| 关 键 词： | 分子生物学 SCAR标记 烟粉虱 温室白粉虱 |
Developing sequence characterized amplified regions (SCARs) to identify Bemisia tabaci and Trialeurodes vaporariorum |
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| CHU Dong;ZHANG You-jun;CONG Bin;XU Bao-yun;WU Qing-jun. Developing sequence characterized amplified regions (SCARs) to identify Bemisia tabaci and Trialeurodes vaporariorum[J]. Plant Protection, 2004, 30(6): 27-30 | |
| Authors: | CHU Dong ZHANG You-jun CONG Bin XU Bao-yun WU Qing-jun |
| Affiliation: | 1.Institute of Vegetables and Flowers;Chinese Academy of Agricultural Sciences. Beijing 100081;China; 2.College of Plant Protection;Shenyang Agricultural University;Shenyang 110161;China |
| Abstract: | The sequence characterized amplified regions (SCARs) for Bemisia tabaci and Trialeurodes vaporariorum were successfully constructed from simple sequence repeats- PCR, respectively. Specific amplification experiments showed that the SCAR markers for B. tabaci were specific to different populations of B. tabaci, and the SCAR markers for T. vaporariorum were specific to different populations of T. vaporariorum . These two markers can be used to identify B. tabaci and T. vaporariorum quickly and accurately. |
| Keywords: | molcular biology SCAR marker Bemisia tabaci Trialeurodes vaporariarum |
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