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| 玉米热激蛋白70基因对温度胁迫的响应 | |
| 引用本文： | 李慧聪,郭秀林,王冬梅,李国良. 玉米热激蛋白70基因对温度胁迫的响应[J]. 河北农业大学学报, 2010, 33(6) |
| 作者姓名： | 李慧聪 郭秀林 王冬梅 李国良 |
| 作者单位： | 1. 河北农业大学,生命科学学院,河北,保定,071001;河北省农林科学院,遗传生理研究所,河北,石家庄,050051 2. 河北省农林科学院,遗传生理研究所,河北,石家庄,050051;河北省植物转基因中心,河北,石家庄,050051 3. 河北农业大学,生命科学学院,河北,保定,071001 |
| 基金项目： | 河北省农林科学院青年基金项目(A09110103) |
| 摘 要： | 利用RT-PCR技术从玉米自交系H21叶片中克隆获得一热激蛋白70(HSP70)基因的完整开放阅读框,全长1 737 bp,编码578个氨基酸,命名为ZmHSP70。编码的氨基酸与其他作物比对分析表明,ZmHSP70与高粱中一推测的蛋白(SbHSP70,XP_002465635)同源性最高,达95%,与水稻中一推测的蛋白(OsHSP70,EAY89062)同源性达87%。半定量RT-PCR的结果表明,该基因明显受42℃热激诱导表达,热激2 h其表达量达最大值;4℃冷胁迫也能诱导ZmHSP70表达量增加,冷胁迫4 h,其表达量达最大值。ZmHSP70是一个受温度诱导的热激蛋白。 |
| 关 键 词： | 玉米 热激蛋白70 温度胁迫 基因表达 |
Responses of HSP70 gene expression to temperature stresses in maize (Zea mays L.) |
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| LI Hui-cong,GUO Xiu-lin,WANG Dong-mei,LI Guo-liang. Responses of HSP70 gene expression to temperature stresses in maize (Zea mays L.)[J]. Journal of Agricultural University of Hebei, 2010, 33(6) | |
| Authors: | LI Hui-cong GUO Xiu-lin WANG Dong-mei LI Guo-liang |
| Affiliation: | LI Hui-cong1,2,GUO Xiu-lin2,3,WANG Dong-mei1,LI Guo-liang2,3(1.College of Life Science,Agricultural University of Hebei,Baoding 071001,China,2.Institute of Genetics , Physiology,Hebei Acadamy of Agriculture , Forestry Sciences,Shijiazhuang 050051,3.Plant Genetic Engineering Center of Hebei Province,China) |
| Abstract: | A heat shock protein 70 gene was cloned from maize(Zea mays L.) using RT-PCR technology,and named as ZmHSP70.The complete open reading frame(ORF) of the gene was 1 737 bp and encoding a protein of 578 amino acid residues.The Blast analysis of the deduced amino acid sequences of the ZmHSP70 with SbHSP70(from Sorghum bicolor) and OsHSP70(from Oryza sativa) showed that this was a highly conserved protein and the identity of the comparison was 95% and 87%.The semi RT-PCR result showed that heat shock at 42 ℃ and cold stress at 4 ℃ induced an increase in the expression level of ZmHSP70.The expression level of ZmHSP70 reached the max at 2 h by heat shock,but the expression level of the gene reached the max at 4 h by cold stress.It indicated that the ZmHSP70 was an induced heat shock protein by temperature. |
| Keywords: | maize HSP70 temperature stresses gene expression |
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