应用固相竞争ELISA、液相阻断ELISA和中和试验检测口蹄疫抗体的比较研究

为了评价建立的口蹄疫固相竞争酶联免疫吸附试验(solid phase competition ELISA,SPC-ELISA)方法与传统的中和试验(VNT)和液相阻断酶联免疫吸附试验(liquid phase block ELISA,LPB-ELISA)检测方法之间的关系,本研究对野外采集的不同种类的猪、牛和牦牛共94份血清样品分别进行O型口蹄疫病毒SPC-ELISA、LPB-ELISA和VNT检测,并分别比较3种方法的符合率和阳性检出率。结果表明,建立的O型口蹄疫病毒SPC-ELISA方法与VNT 具有很好的符合率,达88.3%,其中,猪和牦牛血清符合率高达90%和89.5%,阳性检出率为91.2%比LPB-ELISA更符合VNT,因此建立的SPC-ELISA方法比LPB-ELISA方法更符合VNT。

Comparative Study between New-established FMD Antibody Identification Method SPC-ELISA and Traditional Methods LPB-ELISA and VNT

This study was designed to evaluate the correlation between new-established FMD antibody identification method SPC-ELISA and traditional methods LPB-ELISA and VNT. In this study,94 serum samples (10 from swine,8 from bovine and 76 from yak) which were collected from the field were used to do SPC-ELISA,LPB-ELISA and VNT to detect FMD antibody. Coincidence rate and positive rate were calculated after experiments. The result indicated that,new-established SPC-ELISA had very high coincidence rate with VNT,which was 88.3%. Swine and yak serum’s coincidence rate with VNT were even higher,respectively 90% and 89.5%. Besides,SPC-ELISA also had a higher positive rate than LPB-ELISA,which was more relevance to the positive rate of VNT. The conclusion of the analysis showed new-established FMD antibody detection method SPC-ELISA had greater superiority than traditional method LPB-ELISA.