| ELISA一步法快速检测牛奶中三聚氰胺的研究 |
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| 引用本文： | 赵宁,徐飞,吴小平,王战辉,李杰超,朱景慧,潘净茹.ELISA一步法快速检测牛奶中三聚氰胺的研究[J].中国畜牧兽医,2011,38(4):124-126. |
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| 作者姓名： | 赵宁 徐飞 吴小平 王战辉 李杰超 朱景慧 潘净茹 |
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| 作者单位： | 1. 北京维德维康生物技术有限公司,北京 100085;2. 中国农业大学国家兽药安全评价中心,北京 100193 |
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| 基金项目： | 农业科技成果转化资金项目 |
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| 摘 要： | 在制备三聚氰胺单克隆抗体的基础上建立了一种用于检测牛奶中三聚氰胺含量的间接竞争酶联免疫检测方法,采用一步法操作,评估了方法的回收率、准确度、精密度和特异性,并利用液相色谱―串联质谱法进行了盲样确证试验。结果显示,该方法的线性检测范围为10~810 ng/mL,灵敏度为10 ng/mL,回收率为84.0%~90.0%,同类似物交叉反应率均小于0.1%。本试验建立的ELISA一步法能满足牛奶中三聚氰胺的检测要求。
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| 关 键 词： | 三聚氰胺 牛奶 ELISA 一步法 |
| 收稿时间： | 2010-10-25 |
Study on One-step ELISA Method for Detection of Melamine in Milk |
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| ZHAO Ning,XU Fei,WU Xiao-ping,WANG Zhan-hui,LI Jie-chao,ZHU Jing-hui,PAN Jing-ru.Study on One-step ELISA Method for Detection of Melamine in Milk[J].China Animal Husbandry & Veterinary Medicine,2011,38(4):124-126. |
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| Authors: | ZHAO Ning XU Fei WU Xiao-ping WANG Zhan-hui LI Jie-chao ZHU Jing-hui PAN Jing-ru |
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| Institution: | 1. Beijing WDWK Biotechnology Co.Ltd.,Beijing 100193,China;2. National Center of Veterinary Drug Safety Evaluation,China Agricultural University,Beijing 100193,China |
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| Abstract: | An one-step indirect competitive ELISA system for detection of melamine residues in milk was developed based on monoclonal antibody,and the performance about the detection system such as recoveries,sensitivity,precision and specification were evaluated. The random samples after detected by ELISA had confirmed by LC-MS/MS to access its practicality. The results showed that the calibration curve of this ELISA detection system had the linear detection of 10 to 810 ng/mL,the sensitivity of 10 ng/mL, and the recoveries of 84.0% to 90.0% in milk,with the cross-reactivity within 0.1%. Therefore,the one-step ELISA established in this study is a practical method for determination of melamine residue in milk. |
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| Keywords: | melamine milk ELISA one-step method |
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