| 固态发酵麸皮和玉米芯生产拟康氏木霉（Trichoderma pseudokoningii）孢子的实验研究 |
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| 引用本文： | 孙斐,陈靠山,张鹏英.固态发酵麸皮和玉米芯生产拟康氏木霉（Trichoderma pseudokoningii）孢子的实验研究[J].中国农学通报,2010,26(6):236-239. |
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| 作者姓名： | 孙斐 陈靠山 张鹏英 |
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| 作者单位： | 1. 山东大学生命科学学院,济南,250100
2. 山东大学生命科学学院,济南,250100;山东大学国家糖工程技术研究中心,济南,250100 |
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| 基金项目： | 国家高技术研究发展计划，微生物技术国家重点实验室开放课题基金 |
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| 摘 要： | 本研究以麸皮和玉米芯为主要原料，筛选孢子产量最大的拟康氏木霉固态发酵培养基。采用单因子试验对麸皮、玉米芯比例，培养基含水量，其他佐剂进行了优化。浅盘式固态发酵法进行了模拟发酵。结果表明最优培养基：麸皮、玉米芯按8:2比例混合，调整含水量至55%~60%，添加0.1% KNO3，1%CaSO4，1%蔗糖。在28℃，接种量10%的条件下，利用浅盘式发酵进行拟康氏木霉孢子生产，1千克干料，发酵产出470g分生孢子及菌丝，每克发酵产物含1.34×109孢子。本研究为大规模工业化固态发酵生产拟康氏木霉孢子制剂提供了理论依据。
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| 关 键 词： | 家兔 家兔 子宫内膜细胞 LPS 炎症模型 |
| 收稿时间： | 2009-10-28 |
| 修稿时间： | 2009-11-13 |
The Study of Solid State Fermentation of Trichoderma pseudokoningii Spores with wheat Bran and Corncob |
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| Sun Fei,Chen Kaoshan,Zhang Pengying.The Study of Solid State Fermentation of Trichoderma pseudokoningii Spores with wheat Bran and Corncob[J].Chinese Agricultural Science Bulletin,2010,26(6):236-239. |
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| Authors: | Sun Fei Chen Kaoshan Zhang Pengying |
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| Institution: | Sun Fei1,Chen Kaoshan1*,Zhang Pengying (1College of Life Science,Sh,ong University,Jinan Sh,ong 250100,2 National Glycoengineering Research Center,Jinan Sh,ong 250100) |
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| Abstract: | With the main material ,wheat bran and corncob, the solid state fermentate medium ofTrichoderma pseudokoningii which produces the largest amount of spores was optimized. Single factor experiments were studied on the proportion of wheat bran and corncob, water content, and the other additives content. The optimized medium is composed of wheat bran and corncob with a ratio of 8:2, H2O 55%~60%, KNO3 0.1%, calcium sulfate 1% and sucrose 1%. Shallow tray fermentation experiment was performed, the results show th... |
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| Keywords: | single factor experiments shallow tray fermentation experiment Trichoderma pseudokoningii wheat bran corncob |
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