奶牛瑟氏泰勒虫P33表面蛋白基因的克隆及序列分析

研究根据GenBank上发表的牛瑟氏泰勒虫P33表面蛋白的基因序列设计合成1对引物,用PCR方法扩增出了牛瑟氏泰勒虫的基因片段,将该基因纯化后连接到pMDl8-T栽体上,进行序列测定和分析,并用此方法对延边某奶牛场10头奶牛进行了检测.结果表明:10份样品中9份为阳性,所扩增的目的基因核苷酸长为868 bp,共编码283个氨基酸;该段基因片段与牛泰勒虫韩国株核苷酸序列同源性为99.4%,氨基酸同源性为99.3%.

Cloning and sequence analysis of the dairy COW Theileria sergenti P33 surface protein gene

A pair of primers was designed according to the T. S. gene sequences published in the GenBank. The T.S. gene strain was amplified by PCR. The purified templates DNA were cloned into pMD18 - T vector. The method was used to detect ten cows in the dairy farm. Nine in ten were positive by sequencing aralysis of the T.S. gene. The fragment size was 868 bp, which encodes a protein of 283 amino acids, the homology of nucleotide sequence with Korean strain was 99.4% and 99.3% at amino acid level. This results may be used for expression and studies on vaccine