| 麻雀自然感染鸡传染性法氏囊病病毒的调查 |
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| 引用本文： | 王永山,周宗安,翟春生,王元伦,方元,顾志香,王军,肖元廷. 麻雀自然感染鸡传染性法氏囊病病毒的调查[J]. 中国兽医学报, 1994, 0(3) |
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| 作者姓名： | 王永山 周宗安 翟春生 王元伦 方元 顾志香 王军 肖元廷 |
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| 作者单位： | 南京军区军事医学研究所，南京军区军事医学研究所，南京军区军事医学研究所，南京军区军事医学研究所，南京军区军事医学研究所，南京军区军事医学研究所，山东省淄博市兽医站，83423部队桥头农场 南京210002，南京210002，南京210002，南京210002，南京210002，南京210002 |
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| 摘 要： | 从鸡传染性法氏囊病（IBD）流行的鸡场捕杀麻雀54只,用鸡传染性法氏囊病病毒（IBDV）单克隆抗体夹心阻断ELISA检测抗体,阳性检出率为7.4％(4/54);以逆转录—聚合酶链反应（RT-PCR）检测病毒核酸,阳性检出率为11.1％（6/54）;RT-PCR阳性样本病毒分离亦为阳性。结果表明,IBD流行的鸡场里的麻雀能够发生IBDV自然感染,麻雀可能是IBDV的贮存宿主或二次传染源之一。
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| 关 键 词： | 麻雀 鸡传染性法氏囊病病毒 检测 分离 |
Preliminary Investigation of Sparrows Naturally Infected with Infectious Bursal Disease Virus |
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| Wang Yongshan et al. Preliminary Investigation of Sparrows Naturally Infected with Infectious Bursal Disease Virus[J]. Chinese Journal of Veterinary Science, 1994, 0(3) |
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| Authors: | Wang Yongshan et al |
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| Abstract: | Fifty-four sparrows were captured from a chicken farm where the infectious bursal disease virus (IBDV) was prevalent. The positive rate of antibody to IBDV was 7. 4% (4/54) by monoclonal antibody-sandwich ELISA inhibition, and the positive rate of RNA of IBDV was 11.1% (6/54) by reverse transcribed polymerase chain reaction (RT-PCR). The positive samples of RT-PCR were also positive by isolation of IBDV. The results indicated that the sparrows could naturally be infected with IBDV and might be a reservoir or secondary infection resource of IBDV. |
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| Keywords: | sparrow infectious bursal disease virus detection isolation |
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