利用SRAP分子标记评价小麦三雌蕊近等基因系的遗传背景

普通小麦三雌蕊突变体(TP)具有明显的穗粒数优势,为评估该突变体在育种中的利用价值,进行了近等基因系培育。以三雌蕊突变体(TP)为供体,单雌蕊中国春、川麦28、绵阳29、内麦9号为轮回亲本,经7代回交和4代自交,初步培育出三雌蕊近等基因系CSTP、CM28TP、MY29TP和NM9TP。利用128对SRAP引物对培育的近等基因系及轮回亲本进行遗传分析,结果表明:(1)128对引物共扩增出978条谱带。其中有120对引物的扩增产物具有多态性,占所用引物的93.8%。这120对引物共扩增出638个差异谱带,占总谱带数的65.2%;(2)利用128对SRAP引物计算9个材料之间的遗传相似系数。其中中国春与CSTP的相似系数为0.9346,绵阳29与MY29TP的遗传相似系数为0.9070,川麦28与CM28TP的遗传相似系数为0.9397,内麦9号与NM9TP的遗传相似系数为0.8732;(3)通过聚类分析筛选出2对遗传相似性大于0.93的近等基因系,即CM28TP与川麦28、CSTP与中国春。

EVALUATION ON THE GENETIC BACKGROUND OF WHEAT NEAR ISOGENTIC LINES FOR THREE PISTILS CHARACTER BY SRAP MARKERS

For common wheat line, three pistils (TP), is a valuable mutant trait for wheat breeding, because it could potentially increase the number of gains per spike. In this study, the material with 3 pistils was used as donor parent, and Chinese spring, Chuanmai 28, Mianyang 29 and Neimai 9 were used as recurrent male parents. After successive backcrossing for 7 generations and then self-crossing for 4 generations, 4 near isogentic lines (NILs), CSTP, CM28TP, MY29TP and NM9TP were bred. Genetic similarity and genetic distance of 4 near isogentic lines and their recurrent parents were compared and analyzed by SRAP marker. The results were summarized as follows: (1) All the 128 pairs of SRAP primers amplified 978 bands, 120 pairs (93.8%) of primers produced polymorphic bands and 638 bands (65.2%) were polymorphic; (2) The genetic similarity coefficient of CSTP, CM28TP, MY29TP and NM9TP with their recurrent parents were 0.9346, 0.9397, 0.9070 and 0.8732, respectively. (3) Cluster analysis revealed that CSTP and CM28TP had least difference with the recurrent parent, and was clustered into a small class with the similarity coefficient of 0.93