白僵菌抗多菌灵突变株的诱变及生物学特性研究

采用菌丝生长速率法测定了球孢白僵菌对多菌灵的敏感性和最低完全抑制浓度(MIC)。结果显示,在供试的球孢白僵菌菌株中,Bb06菌株对多菌灵最敏感,其EC₅₀值为1.0688μg/ml,多菌灵对球孢白僵菌菌丝生长的MIC为9μg/ml。通过菌丝块诱变法和分生孢子诱变法获得球孢白僵菌抗多菌灵突变株,并测定球孢白僵菌不同抗性突变株对多菌灵的抗性水平。结果表明,在抗性突变株中,BC-4菌株的EC₅₀值最大,为258.7711,对多菌灵的抗性水平最高,达到242.11;BC-3菌株的EC₅₀值最小,为18.6311,抗性水平最低。同时对球孢白僵菌抗多菌灵突变株的菌丝生长速率和产孢能力差异的研究表明,Bb06菌株(亲本菌株)的生长速率最大,为1.78mm/d,BC-8菌株的生长速率最小,为1.41mm/d。球孢白僵菌不同抗性突变株的产孢能力均比对照敏感菌株强,但不同菌株间存在一定差异,在抗性突变株中,BC-4菌株的分生孢子产生量最大。

INDUCTION AND BIOLOGICAL CHARACTERISTICS OF CARBENDAZIM-RESISTANT MUTANTS OF Beauveria bassiana

The sensitivity and concentration completely inhibited (MIC) of Beauveria bassiana to carbendazim were studied by means of mycelial growth rate method. The results showed that among the tested strains of Beauveria bassiana, Bb06 was the most sensitive to carbendazim, the MIC of Beauveria bassiana to carbendazim was 9μg/ml. The resistant level of the carbendazim-resistant strains of Beauveria bassiana which induced by mycelium and conidia were determined. The results showed that the resistant level of BC-4 was the highest among the carbendazim-resistant strains, and the EC50 and the resistant level were 258.7711 and 242.11, respectively. The resistance level of BC-3 was the lowest among the carbendazim-resistant strains, and the EC50 was 18.6311. The mycelia growth rate and conidia productivity of the carbendazim-resistant strains were also studied. The results showed that the mycelium growth rate of parent strain (Bb06) was the largest(1.78mm/d) and BC-8 was the smallest(1.41mm/d). The conidia productivity of the carbendazim-resistant strains was stronger than the parent strain Bb06. Among the carbendazim-resistant strains, the conidia productivity of BC-4 was the best.