| 免疫层析试纸条(ICS)检测禽流感抗体 |
| |
| 引用本文： | 张喜悦,陈义平,冯娟,刘春菊,吴晓东,王志亮. 免疫层析试纸条(ICS)检测禽流感抗体[J]. 中国兽医学报, 2006, 26(5): 485-487 |
| |
| 作者姓名： | 张喜悦 陈义平 冯娟 刘春菊 吴晓东 王志亮 |
| |
| 作者单位： | 农业部动物检疫所,国家外来动物疫病诊断中心,山东,青岛,266032 |
| |
| 摘 要： | 以纯化的禽流感病毒（avian influenza virus,AIV）核蛋白作为捕捉抗原，金标兔抗鸡抗体作为金标二抗，建立检测禽流感血清抗体的胶体金免疫层析试纸条（immunochromatographic strip, ICS）。对标准阳性血清不同滴度进行检测，ICS试验的敏感性高于琼脂扩散试验（AGP）。特异性试验证实，禽流感的ICS试验与新城疫（ND）、传染性支气管炎（IBV）、鸡传染性法氏囊病（IBD）及减蛋综合症（EDS-76）均无交叉反应。同时，用ICS试验和HI试验检测105份鸡血清，两者具有较好的符合性。结果认为：禽流感ICS具有快速简便、特异、灵敏等优点。
|
| 关 键 词： | 免疫层析试纸条 禽流感 抗体 |
| 文章编号： | 1005-4545(2006)05-0485-03 |
| 收稿时间： | 2005-01-04 |
| 修稿时间： | 2005-01-04 |
Development of Immunochromatographic Strip Test for the Detection of Antibody of AI |
| |
| ZHANG Xi-yue,CHEN Yi-ping,FENG Juan,LIU Chun-ju,WU Xiao-dong,WANG Zhi-liang. Development of Immunochromatographic Strip Test for the Detection of Antibody of AI[J]. Chinese Journal of Veterinary Science, 2006, 26(5): 485-487 |
| |
| Authors: | ZHANG Xi-yue CHEN Yi-ping FENG Juan LIU Chun-ju WU Xiao-dong WANG Zhi-liang |
| |
| Affiliation: | Animal Quarantine Institute, National Exotic Animal Disease Center,Qingdao,Shandong 266032,China |
| |
| Abstract: | We established ani mmunochromatographic strip(ICS) for detecting antibody of avian influenza virus(AIV).TheICS reagent was developed bylabelling antibody rabbit-anti-chicken with colloidal gold and coating Nprotein of AIVon nitrocel-lulose membrane as the capture antigen.The AI ICS was sensitive than AGP when detect their different titer of reference positiveserum.The sera fromthe chickens with NDV,IBV,IBD,EDS-76 were negative in the ICS test.Using ICS and HI test detected105 sera of chicken,the result of ICS was accordant with HI.The ICStest was proved to be rapid,briefness,specificity,and sensi-tivity for detection of antibody of AI. |
| |
| Keywords: | ICS AI Antibody |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
