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| 奶牛子宫内膜粘液抗菌肽的分离纯化 | |
| 引用本文： | 严作廷,米宝明,宗瑞谦,祝秉东,王桂荣,李世宏,王东升.奶牛子宫内膜粘液抗菌肽的分离纯化[J].中国奶牛,2010(7):6-8. |
| 作者姓名： | 严作廷 米宝明 宗瑞谦 祝秉东 王桂荣 李世宏 王东升 |
| 作者单位： | 1. 中国农业科学院兰州畜牧与兽药研究所,兰州,730050 2. 中国农业科学院兰州畜牧与兽药研究所,兰州,730050;甘肃农业大学动物医学院,兰州,730070 3. 甘肃农业大学动物医学院,兰州,730070 4. 兰州大学基础医学院,兰州,730030 |
| 基金项目： | 中央级科研单位基本科研业务费项目 |
| 摘 要： | 为研发奶牛抗菌肽生物制剂,防治奶牛子宫内膜炎,本研究采用酸性尿素聚丙烯酰胺凝胶电泳琼脂糖弥散法检测子宫内膜粘液酸溶性提取物抗菌活性;应用琼脂糖电泳及水平电泳洗脱分离相应的抗菌条带,HPLC进一步分离纯化,琼脂糖弥散法检测纯化分子抗菌活性,Tricine-SDS-PAGE电泳检测其分子量。结果表明,从奶牛子宫内膜酸溶性提取物中纯化出一分子量约为14kD左右的抗菌多肽,对大肠杆菌(E.coliBL21)和金黄色葡萄球菌26003具有抗菌活性。本研究可能分离纯化出一种新的子宫内膜分泌的抗菌多肽,其可能参与奶牛子宫的天然防御机制。 |
| 关 键 词： | 奶牛 子宫内膜 抗菌肽 分离纯化 |
Isolation and Purification of Antibacterial Polypeptide from Cow Uterus Mucus |
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| YAN Zuo-ting,MI Bao-ming,ZONG Rui-qian,ZHU Bing-dong,WANG Gui-rong,LI Shi-hong,WANG Dong-sheng.Isolation and Purification of Antibacterial Polypeptide from Cow Uterus Mucus[J].China Dairy Cattle,2010(7):6-8. | |
| Authors: | YAN Zuo-ting MI Bao-ming ZONG Rui-qian ZHU Bing-dong WANG Gui-rong LI Shi-hong WANG Dong-sheng |
| Institution: | 1.Lanzhou Institute of Animal and Veterinary Pharmaceutics Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050; 2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070; 3.Basic Medical College of Lanzhou University 730030) |
| Abstract: | To research and development biological agents of Dairy Cow antimicrobial peptides, prevent and treat endometritis cow disease, acid-soluble extract of cow uterus mucus was obtained and antibacterial activity was detected by gel overlay technique, then it was further isolated and purified by HPLC and analyzed antibacterial activity by agarose radial diffusion assay. The molecular weight was determined by Trieine-SDS-PAGE. The results showed that the antibacterial polypeptide was able to effectively kill E. coli BL21 and the Staphylococcus bacteria 26003, its molecular weight was about 14kDa. It may be a new antibacterial polypeptide from cow uterus mucus and take part in natural immunology defense in uterus mucus. |
| Keywords: | Dairy cows Uterus mucus Antibacterial peptide Isolation and Purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |