5 - 氨基乙酰丙酸和金雀异黄素促进苹果果皮花青素形成的效应

 无论是树上的苹果果实还是离体果皮, 5 - 氨基乙酰丙酸(ALA) 处理均能显著促进果皮花青素积累, 而金雀异黄素(GNT) 促进离体果皮花青素积累的效应比ALA还明显。光照、ALA和GNT处理均诱导果皮PAL活性短时上升, 表明花青素积累涉及到合成酶活性的诱导。

Promotion of 5-aminolevulinic Acid and Genistein on Anthocyanin Accumulation in Apples

Two plant endogenous substances, 5-aminolevulinic acid (ALA ) and genistein ( GNT) ,were proposed to p romote anthocyanin accumulation of apple fruits in the paper. When 300 mg·L ^{- 1} ALA was app lied to ‘Fuji’apples 43 days before mature harvest, the rate of anthocyanin accumulation and the final content were both almost doubled compared with that of the control. When ALA was applied to the detached fruit discs followed by 48 hour continuous white light irradiation about 160 μmol·m^-2 ·s^-1 , the anthocyanin content rose about 2 times as high as that of the light control, and the latter 2 times as high as that of the dark control. A significant promotion on anthocyanin accumulation was firstly observed in treatment with GNT, where the anthocyanin content in the treated ‘Fuji’ app le discs was about 10 times as high as that of the dark control, about 2 times as effective as ALA treatment. Furthermore, 80 μmol·L ^{- 1} GNT treatment could significantly promote anthocyanin accumulation in‘Golden Delicious’ app le discs, which has seldom been reported before. Additionally, the results showed that promotion ofALA and GNT on anthocyanin accumulation under continuous light was related with induction of phenylalanine ammonialyase ( PAL) activity, because it increased temporarily afterALA and GNT treatments. ALA and GNT are hopeful to be potentially used in fruit production.