井岗霉素在天蓝色链霉菌M145中的异源表达

井岗霉素对水稻纹枯病有良好的防治作用,在我国得到广泛使用。通过结合转移的方法把包含井岗霉素生物合成全基因簇的柯斯质粒9A2导入天蓝色链霉菌M145(Streotpmyces coelicolor M145)中,然后将含有导入质粒的菌株M145/9A2进行发酵,对发酵液进行生物测定的结果显示此菌株发酵液对指示菌水稻纹枯菌有抑制作用,进一步质谱检测结果表明,在天蓝色链霉菌中异源表达井岗霉素成功。

Heterologous Expression of Jinggangmycin in Streotpmyces coelicolor M145

Jinggangmycin has been widely used against sheath blight disease of rice plants in China.The cosmid 9A2 containing the gene cluster for jinggangmycin biosynthesis was introduced into the Streptomyces coelicolor M145 by conjugation.Then the conformed strain M145/9A2 was inoculated into fermentation medium.The antibiotic bioassay experiments showed that the fermentation liquid could inhibit the growth of fungal indicator strain which is sensitive to jinggangmycin.The further analysis by mass spectrometry demonstrated that jinggangmycin has been heterologously expressed in S.coelicolor.