Salvia miltiorrhiza antagonized the alterations of contraction and intracellular calcium of rat ventricular cardiomyocytes induced by anoxia and reoxygenation |
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| Authors: | XU Wan-hong CAO Chun-mei YE Zhi-guo SHEN Yue-liang JIANG Hui-di XIA Qiang |
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| Institution: | 1. Institute of Physiology and Pathophysiology, Hangzhou 310031, China;
2. Department of Physiology, Zhejiang University School of Medicine, Hangzhou 310031, China;
3. Zhejiang University School of Pharmacy, Hangzhou 310031, China |
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| Abstract: | AIM:To study the effect of salvia miltiorrhiza (SM) on cell contraction and intracellular calcium of enzymatically isolated rat ventricular myocytes during normoxia and anoxia/reoxygenation.METHODS:Contraction and intracel ular calcium were determined with video tracking system and spectrofluorometric method,and the chemical anoxic method was employed. RESULTS:The ±dL/dtmax, dL of cell contraction and the amplitude of Ca2+]i in the cardiomyocytes following SM treatment were decreased in a dose-dependent manner. During anoxia, the ±dL/dtmax, dL and amplitude of Ca2+]i were decreased, while the diastolic Ca2+ level was elevated compared with control group. All the contractile parameters and the diastolic Ca2+ level were back toward pretreatment values during reoxygenation, but could not return to control level. After the treatment with SM (3 g/L), ±dL/dtmax and dL of cell contraction and the amplitude of Ca2+]i were higher and the diastolic Ca2+ level was lower than those in anoxia/reoxygenation group. CONCLUSION:SM antagonized effects of anoxia and reoxygenation on cell contraction and intracellular calcium in isolated ventricular myocytes. |
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| Keywords: | Salvia miltiorrhiza Myocardium Anoxia Intracellular calcium Rats |
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