Changes of Ca2+ activated potassium channels and cellular-proliferation in autogenous vein grafts |
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Authors: | Changes of Ca SONG Sheng-yun MA Bao-an FAN Qing-yu |
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Affiliation: | 1. Department of Orthopedics Oncology Institute and Orthopedics Surgery Center of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China;2. Department of Surgery, Baoshan Hosptial of Yunnan Genaral Armed Polices Forces, Baoshan 678000, China |
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Abstract: | AIM: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. METHODS: The contraction of venous ring was measured by means of perfusion in vitro. The intimal proliferation and proliferation of cultured smooth muscle cells (vascular smooth muscle cells, VSMCs) were observed by the means of computerised image analysis and MTT method, respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. RESULTS: 1 week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they were more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control). TEA (blocker of Ca2+ activated potassium channels) increased MTT A490 value of VSMCs from femoral vein in a dose-dependent manner (P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts 1-4 weeks after transplantation (P<0.05, n=5). CONCLUSION: KCa was inhibited in autogenous vein graft, which accounted for vasospasm and intimal proliferation. |
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Keywords: | Transplantation autologous Veins Vasospasm Ca2+ activated potassium channel |
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