Role of endogenous nitric oxide in apoptosis of alveolar epithelial cells in the development of pulmonary fibrosis in rats |
| |
| Authors: | CHEN Xiao-ling SHEN Tie-bao ZHAO Song LI Ying-min ZHANG Ai-zi LI Wen-bin AI Jie |
| |
| Affiliation: | 1. Department of Pathophysiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China;2. Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China;3. Department of Histology & Embryology, Hebei Medical University, Shijiazhuang 050017, China |
| |
| Abstract: | AIM: To study the role of high level of endogenous nitric oxide (NO) in apoptosis of alveolar epithelial cells in the development of pulmonary fibrosis in rats. METHODS: The content of nitrite/nitrate (NO2-/NO3-) in out-flowing pulmonary blood (OPB) was assayed by nitric acid reduction method. The apoptosis of alveolar epithelial cells was observed by TdT-mediated dUTP nick-end labeling (TUNEL) and electron microscopy, respectively. The above indices were observed on the day 14 and the day 30 after intratracheal administration of BLMA5 alone or along with blockade of iNOS by aminoguanidine (AG) in rats. RESULTS: (1) Both the content of NO2-/NO3- in OPB and the number of apoptotic alveolar epithelial cells in lung were increased in BLMA5 14 d group, compared with normal control group and BLMA5 30 d group, respectively (P<0.05). The high level of NO2-/NO3- in OPB and the apoptosis of alveolar epithelial cells were ameliorated by AG. CONCLUSION: The apoptosis of alveolar epithelial cell is induced by high level of endogenous NO in the development of pulmonary fibrosis. |
| |
| Keywords: | Pulmonary fibrosis Alveolar epithelial cells Apoptosis Nitric oxide Rats |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
|
点击此处可从《园艺学报》下载全文 |
|
