Proteomic analysis of the effects of tumor necrosis factor-α on endothelial cells |
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Authors: | L Jun-ping WANG Shu-ren MA Zeng-chun WANG Sheng-qi |
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Affiliation: | 1. Department of Pathophysiology, Sichuan University, Chengdu 610041, China;2. Institute of Radiation Medicine Sciences, Academy of Military Medical Sciences, Beijing 100850, China |
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Abstract: | AIM: To investigate the affected proteins by tumor necrosis factor (TNF)-α in endothelial cells, and further explore the potential molecular mechanism of TNF-α on endothelial cells. METHODS: Nitric oxide (NO) production in the cultured human umbilical vein endothelial cells (HUVECs) was measured by a NO assay kit. Proteomic alterations were analyzed using two-dimensional electrophoresis, and peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. RESULTS: NO production in HUVECs decreased significantly after TNF-α treatement. Proteomics analysis showed 21 protein spots were changed including 9 spots that were increased and 11 spots that were decreased after TNF-α stimulation, and 1 spot was only detected in TNF-α activated cell gels. CONCLUSIONS: Decreased the expression of ecNOS by TNF-α might result in decreased NO production. Up-regulated MAP/ERK kinase 3 expression might imply that TNF-α activates the expression of adhesion molecules. Cytoskeletal protein actin is also involved in TNF-α injuried HUVECs. Proteomic analysis can find some clues for identifying new potential target of TNF-α. |
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Keywords: | Tumor necrosis factor Proteome Endothelial cells Nitric oxide |
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