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Influence of the serum of Radix Ophiopogonison the apoptosis related gene expression and intracellular Ca2+ in vascular endothelial cells treated with LPS |
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| Authors: | ZHANG Xu ZHANG Chao-ying WANG Wen XU Dong-qing YANG Jin |
| Institution: | 1. Basic Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China; 2. College of Life Science, Nanjing, Normal University, Nanjing 210024, China |
| Abstract: | AIM:To explore the molecular mechanism of Radix Ophioponis against vascular endothelial cell (VEC) apoptosis induced by LPS.METHODS:The apoptosis of human umbilical vascular endothelial cells(HUVEC) was induced by lipopolysaccharide(LPS). The influence of Radix Ophiopogonis on the expression of bcl-2 and intracellular Ca2+ was detected by flow cytometry and laser confocal microscope.RESULTS:The serum containing Radix Ophiopogonis suppressed the increase in bcl-2 expression and overloading of Ca2+ induced by LPS in HUVEC.CONCLUSION:The mechanism of Radix Ophiopogonis against HUVEC apotosis may be related with its regulatory effect on bcl-2 expression and remission of Ca2+ overloading. |
| Keywords: | Radix Ophiopogonis Vascular endothelium Calcium Genes bcl-2 |
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