The consensus oligonucleotide competitive binding with NF-κB inhibits TNF production

AIM:To investigate whether a consensus oligonucleotide, which contains nuclear factor-kappa B(NF-κB) binding site, decreases TNF production stimulated by lipopolysaccharide(LPS).METHODS:The thiophosphoric acid modified oligonucleotides were transferred into rat peritoneal macrophages directly, TNF content in LPS-stimulated cell culture supernatant was determined by ELISA.RESULTS:Adding the consensus oligonucleotides with NF-κB binding site into macrophages markedly decreased TNF production following LPS stimulation.CONCLUSION:These results show that use of the consensus oligonucleotides with NF-κB binding site, which can combine with NF-κB, can block or decrease TNF production induced by LPS in macrophages.