Antitumor effect of chlorophyllin in vitro |
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Authors: | DING Xiao-wen ZHENG Shu PENG Jia-ping LV Qing-hua HUANG Jian |
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Affiliation: | Cancer Institute, Zhejiang University, Hangzhou 310009, China |
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Abstract: | AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC50 value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G1 phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibited the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G1 phase. |
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Keywords: | Chlorophyll Telomerase Colorectal neoplasms Apoptosis |
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