Effects of angiotensin II and losartan on collagen synthesis in rat hepatic stellate cells |
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Authors: | ZHANG Yi-jun YANG Xi-shan WU Ping-sheng ZHANG Xiao-feng CHEN Xiao-qing ZENG Jian-ying |
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Institution: | 1. Department of Gerontology, Liuhua Bridge Hospital, Guangzhou 510010, China;
2. PLA Institute of Digestive Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;
3. Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China |
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Abstract: | AIM: To investigate the effects of angiotensin II (Ang II) and AT1a receptor antagonist (losartan) collagen synthesis in rat hepatic stellate cells (HSCs). METHODS: ① Rat HSCs were isolated, cultured and identified. ② Rat HSCs were incubated in the medium with different concentrations of AngII or losartan, then the quantity of collagen was examined by -proline release assay. RESULTS: ① The yield of HSCs was 2×107-3×107/per rat, their viability and purity was more than 95% and 90%, respectively. ② The yield of collagen in HSCs significantly got a rise in a concentration-dependent manner when HSCs were incubated with AngII (10-6mol/L-10-10 mol/L) (P<0.05). While HSCs were influenced by the antagonist of AT1a (10-6 mol/L-10-9 mol/L), the quantity of collagen dropped greatly (P<0.05). CONCLUSIONS: Ang II stimulates HSCs to produce more collagen. Losartan inhibits the cell to synthesize collagen via AT1a receptor (P<0.05). The results indicate that Ang II may play an important role in the development of hepatic fibrosis, and using AT1a antagonist may offer a new strategy to prevent hepatic fibrosis. |
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Keywords: | Angiotensin II Losartan Liver cirrhosis |
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