Role of NF-κB in inducing HEL cell proliferation and apoptosis during human cytomegalovirus infection |
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Authors: | WANG Hui WEN Liang-zhen LING Xia-zhen |
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Affiliation: | Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical University, Wuhan 430030, China |
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Abstract: | AIM:To investigate whether human cytomegalovirus(HCMV) regulate human embryonic lung fibroblast(HEL) cell proliferation and apoptosis by activating NF-κB.METHODS:Immunohistochemistry and Western blot analysis were used to detect the NF-κB translocation and/or Bcl-2 and the levels of I-κBα during HCMV infection. Apoptotic cell were examined by flow cytometry, and the HEL cell proliferation was determined by MTT.RESULTS:The levels of NF-κB in the nucleus reached highly 48 h postinfection, and the levels of I-κBα were low 24 h postinfection. The activity of NF-κB was inhibited 120 h postinfection. The levels ofbcl-2was accorded with the activity of NF-κB. HCMV promoted HEL cells to proliferate before 72 h postinfection and induced apoptosis 120 h postinfection.CONCLUSION:NF-κB plays a role in HEL cell proliferation and apoptosis during HCMV infection, and it involves in the pathological mechanisms of diseases associated with HCMV infection. |
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Keywords: | Cytomegaloviruses Apoptosis Genes bcl-2 NF-κB |
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