Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat |
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Authors: | ZHANG En-guang ZHANG Huai-qin LIN Jie HUANG Wei-jian LI Shang-gong XU Li-xin |
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Institution: | Department of Cardiology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325003, China |
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Abstract: | AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P<0.01), 2.3 folds at 2 nd week(P<0.01) and 1.7 flods at 4 th week(P<0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P<0.01), 1.9 flods(P<0.01) and 1.4 folds(P<0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling. |
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Keywords: | Matrix metalloproteinases Myocardial ischemia Ventricular remodeling Rats |
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