Antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes |
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| Authors: | LIU Ying-ge QI Hao-wen LI Huan-zhang SU Ming-quan YU Wen-bin MA Yue-yun |
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| Affiliation: | 1. Department of Respiratory Medicine, Fourth Military Medical University, XI'an 710033, China;2. Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, XI'an 710033, China |
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| Abstract: | AIM: To observe the antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes. METHODS: Rat thymus lymphocytes were separated by Ficoll-Urografin density gradient centrifugation. Lipofectin was used to introduce antisense, sense and mismatched oligonucleotides for c-myc to rat thymus lymphocytes. The antiproliferative effect was assayed by incorporation of [3H]-TdR and MTS cell proliferation assay. TR-PCR was used to detect the expression of c-myc mRNA. RESULTS: c-myc antisense oligonucleotide inhibited ratthymus lymphocytes proliferation[(0.14±0.03)A vs(0.32±0.16)A,P<0.05],but this ef ect had no relationship with the concentration of c-myc antisense oligonucleot ide.c-myc antisense oligonucleotide decreased the expression of c-myc mRNA in rat thymus lymphocytes. CONCLUSION: c-myc antisense oligonucleotide inhibited rat thymus lymphocyte proliferation. |
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| Keywords: | Genes myc Oligonucleotides antisense Lymphocytes |
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