Effect of anti-CD59 on CVF-induced platelet activation

AIM:To study the reactions of human platelet to active complement and the effects of anti-CD59 on human platelet activation induced by complement.METHODS:By applying CVF to activate complement, the platelet aggregation and release reactions induced by activated complement with or without appling anti-CD59 with different doses to block the complement modulative protein CD59 in healthy individuals, were observed.RESULTS:CVF induced platelet release and significant and lasting metamorphosis in healthy individuals, but platelet aggregation was not observed. CVF-induced platelet metamorphosis showed positive linear correlation to lg concentration of CVF (r=0.970. P<0.01. n=36). Anti-CD59 enhanced CVF-induced platelet shape change with a dose-dependent manner. The max enhancive ratio of platelet shape change was 1.36(P<0.01). Anti-CD59 enhanced platelet ATP release induces by CVF.CONCLUSION:Complement activated by CVF induces significant and lasting platelet metamorphosis and release reaction, but dose not induce platelet aggregation in healthy adult males. Anti-CD59 promotes the platelet reactions induced by active complement.