Effect of crystallin βB2 on the aging of lens |
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| Authors: | LI Wen-jie Joseph-Fu S-C |
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| Institution: | 1. Central Laboratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Department of Biochemistry and Moleculor Biology, New Jersey Medical School and Graduate School of Medical Sciences, Newark NJ 07103, USA |
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| Abstract: | AIM: To study the effect of crystallin βB2 on the aging of lens. METHODS: SD rats were maintained routinely and killed at 6 different ages (1 d, 8 d, 2 weeks, 8 weeks, 8 months and 1.5 years). Water-soluble crystallins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis (IEF/SDS-PAGE). After Comassize blue staining, the crystallin patterns were screened and analyzed. βB2 crystallin and the main chaperone proteins (αA2, αB2) were identified and the relative quantity was measured. RESULTS: (1) The quantity of water-soluble crystalline βB2 increased in close relation to the aging of the rat. (2) αA2, αB2 chaperone proteins increased with the aging of the rat too. (3) The change of the quantity of water-soluble crystalline βB2 was closely related to the changes of αA2 , αB2 chaperone proteins. (4) Degraded and modified crystallins began to appear clearly in the lens after 8 months old. CONCLUSION: Based on our results, we infer that water-soluble crystalline βB2 increases with the aging dof the rat, which is helpful to maintain the structure and transparency of the lens. |
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| Keywords: | Crystallins Aging Lens |
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