Changes in the expression of endothelin system in rats with chronic heart failing |
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Authors: | QU Wei WANG Zi-zheng WANG Shu-kui HUANG Ming |
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Institution: | 1. Nanjing First Hospital Affiliated of Nanjing Medical University, Center Laboratory,Nanjing 210006, China;
2. China Pharmaceutical University, Research Division of Pharmacology, Nanjing 210009, China |
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Abstract: | AIM: To study the changes of endothelin system during chronic heart failure (CHF) and imply the relationship between endothelin system and the course of CHF by observing the mRNA expression of endothelin receptors (ETAR and ETBR) and PreproET1 in early stage and later stage of CHF caused by left coronary artery ligation. METHODS: The mRNA expression of ETA, ETB receptors and PreproET1 were detected by RT-PCR technique. The plasma concentrations of ET1 and ANP were determined by RIA method. RESULTS: The plasma concentrations of ET1 and ANP, and the mRNA expression of ET receptors and PreproET1 in the lefe ventricle increased significantly in early stage (myocardial infarction 10 d). While the plasma concentrations of ET1 and ANP in later stage (myocardial infarction 70 d) were higher than those in the early stage. However, the mRNA expression of ETAR, ETBR and PreproET1 decreased significantly. The mRNA expression of ETAR in myocardial infarction (MI) 70 d rats had no difference with those in sham-operated rats and the mRNA expression of ETBR and PreproET1 in MI 70 d rats was lower significantly than those in MI 10 d rats, but significantly higher than those in sham-operated rats. CONCLUSION: The changes of ET receptors and PreproET1 mRNA expression are involved in the cardiac function modulation during the different stages in chronic heart failure. |
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Keywords: | Heart failure congestive Receptors endothelin Rats |
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