Effects of recombinant viral chemokine vMIP-II on cellular immunity stimulated by LPS

AIM: To investigate the produce of intracellular cytokine following short-term in vitro stimulation with vMIP and LPS, and discuss the effect of vMIP to cellular immunity. METHODS: The methods of Cross-linking of radioactivity, ELISA and four-colors flow cytometer were used to test the level of the secretion of chemokine IL-12 and intracellular cytokine IFN-γ and IL-4. RESULTS: After treated the PBMCs with vMIP-II, the levels of secretion of IL-12, IFN-γ and IL-4 were reduced in the present of LPS by competitively combining chemokine receptor; vMIP promoted CD4⁺T cell to secrete IL-12, IFN-γ and IL-4. CONCLUSION: vMIP-II can protect systemic response of immunity and reduce extremely inflammation by down-regulating proinflammation.