The related gene expression in mesenchymal stem cells differentiating to neuron-like cells |
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Authors: | FENG Ying XIE Fu-kang HU Li-ping ZHANG Zhi ZHOU Xiao-qiu |
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Affiliation: | Department of Histology and Embrology, Sun Yat-sen university, Guangzhou 510080, China |
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Abstract: | AIM: To analyze neuron-related gene expression before and after mesenchymal stem cells (MSCs) differentiating into neuron-like cells. METHODS: MSCs were induced to neuron-like cells with β-mercaptoethanol. Before inducement and at 8 h after inducement, the total RNA was extracted, then the expression of microtubule-associated protein-2 (MAP-2), growth -associated protein -43 (GAP-43), NSE, nestin and neurofilament (NF) mRNA were detected with RT-PCR. RESULTS: NSE mRNA expressed before and after inducement, MAP-2, GAP-43, nestin and NF mRNA only expressed after inducement. CONCLUSION: The differentiation of MSCs into neuron-like cells may be related to MAP-2, GAP-43, nestin and NF expression. |
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Keywords: | Stem cells Differentiation Gene expression Neurons Mercaptoethanol |
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