Effects of hemorrhagic shock on BKCa channel tyrosine phosphorylation level in mesenteric artery in rats |
| |
Authors: | ZHOU Rong LIU Liang-ming HU De-yao |
| |
Institution: | Second Department of Research Institute of Surgery, Daping Hospital, Third Militory Medical University, Chongqing 400042, China |
| |
Abstract: | AIM: To study the effects of hemorrhagic shock on BKCa channel tyrosine phosphorylation in rat superior mesenteric artery and the role of nitric oxide (NO) in BKCa channel tyrosine phosphorylation. METHODS: The hemorrhagic shock model (35±5)mmHg] was established in rats and the whole lysate of superior mesenteric artery were extracted and analyzed by immune precipition (IP) and immunoblotting. The tyrosine phosphorylation levels of BKCa channel alpha-subunit in mesenteric artery in hemorrhagic shock rats were investigated, and the modulation of BKCa channel alpha-subunit tyrosine phosphorylation by NO and its dose-and time-dependended relationships were observed. RESULTS: The tyrosine phosphorylation level of BKCa channel alpha-subunit in mesenteric artery in rats increased significantly after hemorrhagic shock 2 h and 4 h (P<0.01 ), and L-arginine (5×10-5-5×10-4 mol/L) up-regulated BKCa channel alpha-subunit tyrosine phosphorylation in primary cultured VSMC in a 30 min incubation and without significant decrease after 2 h; L-arginine induced BKCa channel alpha-subunit tyrosine phosphorylation in a dose-dependent manner. CONCLUSION: Hemorrhagic shock enhances BKCa channel tyrosine phosphorylation in resistant artery in rats, and NO is involved in this process. |
| |
Keywords: | Shock hemorrhagic BKCa channels Tyrosine phosphorylation |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|