Effect of salidroside on mitochondrial membrane potential during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells |
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Authors: | ZHANG Wen-sheng ZHU Ling-qun DENG Rui-chun NIU Fu-ling TIAN Rong |
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Institution: | 1. Institute of Natural Medicine & Chinese Medicine Resources, College of Resources Science & Technology, Beijing Normal University, Beijing 100088, China;
2. The Key Laboratory of Chinese Internal Medicine of Ministry of Education China, Dong Zhi Meng Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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Abstract: | AIM: To investigate the effects of salidroside on intracellular free calcium concentration Ca2+]i, apoptosis, mitochondrial membrane potential (MMP) and activity during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells. METHODS: Mitochondrial activity was measured by methylthiazolyl tetrazolium test. MMP,Ca2+]i and apoptosis were measured by flow cytometry. RESULTS: SH-SY5Y cells were cultured in a hypoxia/hypoglycemia condition for 2, 4, 6 and 12 h,Ca2+]i and apoptosis rate significantly increased compared with control group (P<0.01). After hypoxia /hypoglycemia cultures, MMP and mitochondrial activity declined 29.17% (P<0.01) and 38.80% (P<0.01) at 2 h, 56.72% (P<0.01) and 63.58% (P<0.01) at 12 h, were lower than that in control group (P<0.01). Salidroside significantly decreased Ca2+]i and apoptosis rate, and increased MMP and mitochondrial activity in hypoxia /hypoglycemia-treated SH-SY5Y cells. CONCLUSIONS: Salidroside might inhibit the decline in MMP and mitochondrial activity induced by hypoxia /hypoglycemia, and has an inhibitory effects on neuronal apoptosis. The mechanism might be related to inhibiting intracellular calcium overload. |
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Keywords: | Salidroside SH-SY5Y cells Mitochondria Membrane potentials Apoptosis Anoxia Hypoglycemia |
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