Acute pulmonary injury caused by echinococcus granulosus cyst fluid

AIM:to establish an animals model of pulmonary embolism caused by echinococcus granulosus (E.g)cyst fluid.METHODS:Cyst fluid were isolated from sheep liver . Twenty-one rabbits were randomized into 3 groups: group Ⅰ(saline group), group Ⅱ(clear cyst fluid group), group Ⅲ (sand-contained cyst fluid group). Operation was performed on each animal to place a femoral artery catheter and a femoral vein catheter. Saline, clear cyst fluid or sand-contained cyst fluid were given to rabbits at a dose of 3 mL/kg body weigh . Observations were then made at 10, 30, 60 minutes to determine the changes of MAP, blood gas and vaso-active substance. Then ECT scanning image was obtained .After observation, all animals were killed and the lungs were harvested for histological examination by light microscopy.RESULTS:Remarkable decline of PaO₂, MAP and rise of TXB₂, 6-keto-PGF_1α were observed in group Ⅲ and group Ⅱ(P＜0.05). ECT examination showed radioactivity defect in group Ⅲ and radioactivity weakening of the lung in group Ⅱ. Histological examination of the lungs showed that sand-contained cyst fluid may cause an "ARDS-like" change.CONCLUSION:Animals in group Ⅲ basically simulated the clinical situation of pulmonary embolism caused by E.g cyst content. The sand contained in the fluid played an important role in damaging the lung. Vaso-active substance had relevance to the lung injury.