Inhibitory effect of polypeptide from Buthus martensii Karsch on neoplasm cells |
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Authors: | KONG Tian-han LIN Shan HAN Xue-fei JU Ji-hang DONG Wei-hua |
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Affiliation: | 1. Institute of Snake Venom Research, Guangzhou Medical College, Guangzhou 510182, China;2. Department of Oncology, The Wujing Hospital of Hangzhou City, Hangzhou 310015, China;3. Center of Venom and Biological Toxins, Henan Medical University, Zhengzhou 450052, China;4. Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China;5. Department of Pathophysiology, Guangzhou Medical College, Guangzhou 510182, China |
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Abstract: | AIM: To observe the inhibitory effects of polypeptide from Buthus martensii venom (PBMV) on human tumor cell lines and the influence of PBMV on cell cycle migration, expression of tumor-suppressor gene p53 and the membrane potential of CNE-2Z cells. METHODS: MTT colorimetric method, the colony formation and mitosis index, flow cytometry assay and intracellular recording with glass microelectrodes were used. RESULTS: PBMV had obvious cytotoxicity on several tumor cell lines. The cells grow was inhibited by PBMV, colony formation rate and mitotic index of tumor cells were reduced and the number of polykaryocyte was decreased. CNE-2Z cells in S phase were reduced evidently after they were treated with PBMV (P<0.01). After CNE-2Z cells were incubated with PBMV, P53 protein expression in the cells decreased obviously. Differences were very distinct (P<0.01). The negative potential of cell membrane reduced evidently and cell membrane was in depolarization state. CONCLUSION: PBMV probably possesses the effects of the "membrane toxin". By decreasing the membrane potential, the growth and proliferation of tumor cells were interfered by PBMV. |
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Keywords: | Scorpion venoms Eca109 cel Cell cycle Protein P53 Membrane potentials |
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