Effect of antisense oligonucleotides of c-sis on cellular cycle and proliferation of vascular smooth muscle cells in pulmonary artery |
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Authors: | LIU Han-min XIONG Ying YUAN Li-xing LI Mi LUO Chun-hua |
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Institution: | Department of Pediatrics, the Second West China Hospital, Sichuan University, Chengdu 610041, China |
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Abstract: | AIM:To investigate the effect of antisense oligonucleotides(ASON) of c-sis on cellular cycle and proliferation of pulmonary artery vascular smooth muscle cells(VSMC).METHODS:Tissue mass culture was done to get VSMC of pulmonary artery. Different concentrations of antisense oligonucleotides of c-sis were added into the cultures to observe the VSMC proliferation curve using MTT test. The changes of VSMC cellular cycle were also observed by flow cytometry.RESULTS:ASON with mid-to high concentrations restrained the proliferation of VSMC apparently with the peak of cell growth being attenuated or eliminated. Affected by mid-concentration ASON, PDGF-BB showed significant accelerating effect on the proliferation of VSMC. The ratio of G0/G1 in cellular cycle was increased significantly in VSMC culture with ASON in comparison with control. The G0/G1 ratio also showed significant differences among different concentration of ASON groups(P<0.05).CONCLUSION:Mid-to high concentration of ASON was a powerful inhibitor of cellular proliferation for pulmonary artery VSMC. ASON increased the ratio of G0/G1 significantly and the increase seems to be ASON dosage dependent. |
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Keywords: | Proto-oncogene protein c-sis Oligonucleotides antisense Muscle smooth vascular Cell cycle |
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