面包酵母B_(188)发酵豆渣制备单细胞蛋白的研究

目的]提升豆渣的饲用价值,缓解饲料短缺矛盾.方法]以豆渣为培养基主要原料,对面包酵母B_(188)固体发酵生产单细胞蛋白(SCP)的工艺条件进行了研究.结果]通过对培养基组成和培养条件的优化,确定了豆渣固体发酵生产单细胞蛋白的工艺条件;其优化工艺是麸皮与豆渣的最佳配比为1∶6;尿素、硫酸铵的添加量分别是3;,0.2;;起始pH 5.0,接种量12;,培养温度30℃,培养时间48 h;在此优化条件下,SCP合成量达最大值,蛋白质含量为36.19;.结论]该研究为豆渣的饲料化规模生产提供了一种参考方法.

Studies on SCP Preparation by Saccharomyces cerevisiae B_(188) Using Bean Dregs

Objective and Metnod]The objective of this paper was to investigate the technological conditions of SCP producton by Saccharomyces cerevisiae B_(188) to improve the feeding value of bean dregs and to relieve the shortage of forage by use of the bean dregs as raw medium.Result]Optimization of the mediums and the cultivating conditions and the technology was determined.The ratio of bran and bean dregs was 1∶6,the initial pH 5.0,ammonium sulfate and added amount were 3.0% and 0.2% respectively,the inoculation volume were 12%,the cultivate temperature was 30℃and the period for 48 h.In the optimum conditions, the content of SCP synthetic was up to the maximum, protein content was 36.19%.Conclusion]The technology provides a good basis for large scale production of bean dregs feed.