玉米自交系B73全基因组cystatin抗虫基因家族分析

半胱氨酸蛋白酶抑制剂(cystatin)基因具有cystatin结构特征的蛋白结构域,广泛存在于植物或动物体内。此类基因在植物或动物蛋白质组中具有多个功能各异的家族成员,并在抗虫中扮演重要角色。通过利用玉米B73全基因组的基因表达序列数据,对12个cystatin基因的结构组成、染色体分布、系统进化等进行了分析。鉴定了该基因家族在蛋白序列间3个高度保守基序,构建了基因家族系统进化树,为该重要基因家族的进化提供了依据的同时也为克隆和分离该类抗虫基因提供参考。

Genome-Wide Analysis of cystatin Gene Family in Maize Inbred Line B73

Cystatin genes are cysteine proteinase inhibitors with a protein pattern of cystatin-containing domain, which exist widely in many proteins from plants and animals. This protein has many different family members distributing in plant and animal proteome,and plays key roles in plants against pests. Here,we identified com- plete set of 12 different cystatin gene in the maize（Zea mays L. ）inbred line B73 genome. The putative cystatin genes were characterized with respect to structural diversity, chromosome distribution, phylogenetic relationship and so on. Three conserved motifs were found among proteins of cystatin gene of maize. Phylogenetic analysis of these proteins indicated that four groups exist. The results of this study will provide evidence for evolution for cystatin gene family in maize and benefit for separating and cloning resistance gene to defend the insects.