| 蜡蚧轮枝菌昆明菌株发酵培养技术研究 |
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| 引用本文： | 张亚平,周天雄,杨美林.蜡蚧轮枝菌昆明菌株发酵培养技术研究[J].云南农业大学学报,2007,22(4):601-603. |
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| 作者姓名： | 张亚平 周天雄 杨美林 |
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| 作者单位： | 云南农业大学植物保护学院 云南昆明650201 |
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| 摘 要： | 用5种培养液对蜡蚧轮枝菌昆明菌株（KM9803）进行液体培养的结果:以改良的沙氏培养液和豆粉-土豆汁培养液中芽生孢子产量较高；摇瓶时间以4～5d较适宜。产孢量可以达到7.26×108～9.09×108个芽生孢子/mL; 固体培养阶段培养基：加水量以1∶0.6为宜，固体培养发酵时间以7d为宜,分生孢子产量可达到4.05×109～4.11×109孢子/g。
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| 关 键 词： | 蜡蚧轮枝菌 孢子浓度 培养液筛选 |
| 文章编号： | 1004-390X（2007）04-0601-03 |
| 收稿时间： | 2006-11-24 |
| 修稿时间： | 2006-11-24 |
Study on Fermentation and Culture Technology of Verticillium lecanii |
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| ZHANG Ya-ping,ZHOU Tian-xiong,YANG Mei-lin.Study on Fermentation and Culture Technology of Verticillium lecanii[J].Journal of Yunnan Agricultural University,2007,22(4):601-603. |
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| Authors: | ZHANG Ya-ping ZHOU Tian-xiong YANG Mei-lin |
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| Institution: | Faculty of Plant Protection, Y A U, Kunming 650201, China |
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| Abstract: | Blastospore yields of KM9803 in 5 kinds of liquid mediums shows that high blastospore yields can be achieved in SDAY and potato liquid, it’s about 7.26×108～9.09×108 blastspore/mL and just about 4～5 days for fermentation; Medium: water is 1.0 to 0.6. The conidiophore yields of KM9803 in solid medium can reach to 4.05×109～4.11×109 conidiophore/g and need 7d for fermentation. |
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| Keywords: | Verticillium lecanii concentration of blastospore liquid medium-selection |
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