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| 落叶松扦插生根过程抑制消减杂交文库构建及分析 | |
| 引用本文： | 冯健,齐力旺,孙晓梅,韩素英,张守攻.落叶松扦插生根过程抑制消减杂交文库构建及分析[J].林业科学研究,2009,22(5):672-676. |
| 作者姓名： | 冯健 齐力旺 孙晓梅 韩素英 张守攻 |
| 作者单位： | 1. 中国林业科学研究院林业研究所细胞生物学实验室,北京,100091;辽宁林业科学研究院,辽宁,沈阳,110032 2. 中国林业科学研究院林业研究所细胞生物学实验室,北京,100091 3. 中国林业科学研究院森林生态环境与保护研究所,北京,100091 |
| 基金项目： | 国家"863"计划"高产优质多抗林木花草分子与细胞高效育种技术及品种创制(2006AA100109)"和国家转基因与产业化专项"优质、杂种落叶松抗旱基因工程育种研究(J2002-B-005)" |
| 摘 要： | 以落叶松扦插生根率存在显著差异的2个全同胞优良无性系茎和根为材料,应用抑制消减杂交技术构建了扦插生根过程中2个差异表达cDNA文库,酶切效率检测、连接效率检测、差减效率检测结果表明:这2个落叶松扦插生根过程差异表达cDNA文库构建成功.利用PCR扩增和点杂交试验筛选阳性克隆,将阳性克隆测序,并经生物信息学分析,最终获得521个UniEST. |
| 关 键 词： | 落叶松 抑制消减杂交文库 扦插 |
| 收稿时间： | 3/7/2008 12:00:00 AM |
Construction and Analysis of the Suppression SubtractiveHybridization L ibrary of Larix Cuttings during Rooting |
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| FENG Jian,QI Li-wang,SUN Xiao-mei,HAN Su-ying and ZHANG Shou-gong.Construction and Analysis of the Suppression SubtractiveHybridization L ibrary of Larix Cuttings during Rooting[J].Forest Research,2009,22(5):672-676. | |
| Authors: | FENG Jian QI Li-wang SUN Xiao-mei HAN Su-ying and ZHANG Shou-gong |
| Institution: | Group of Trees Cell Biology, Research Inst itu te of Forestry, CAF, Beijing 100091, Ch ina; Liaon ing A cad emy of Forestry,Shenyang 110032, L iaon ing, Ch ina;Group of Trees Cell Biology, Research Inst itu te of Forestry, CAF, Beijing 100091, Ch ina;Group of Trees Cell Biology, Research Inst itu te of Forestry, CAF, Beijing 100091, Ch ina;Research Inst itu te o fForest Eco logy, Environment and Protect ion, CAF, Beijing 100091, Ch ina;Group of Trees Cell Biology, Research Inst itu te of Forestry, CAF, Beijing 100091, Ch ina |
| Abstract: | Taking the stem s and roots of tw o fu ll sib clones w ith significant d ifference in roo ting rate as testmaterials, tw o differential expression cDNA librar ies o f Larix cutting during root ing w ere established by PCR se lectTM cDNA subtract ion k i.t The resu lts of analysis of Rsa I d igest ion, ligat ion and subtraction eff iciency show edtha t the tw o libraries w ere established successfully. The posit ive clones w ere screened by PCR and dot blots. 521UniEST w ere obta ined by sequenc ing and b io informat ics ana lysis. |
| Keywords: | Larix suppression subtract ive hybrid izat ion library cutting |
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