酶联免疫测定技术在研究氟虫腈降解中的应用

利用单克隆抗体间接竞争ELISA技术检测氟虫腈在青菜和土壤中的降解规律。其在青菜中的降解动态符合一级降解动力学方程C=0.1927e-0.0666t,降解半衰期为10.4d,r=-0.9787;在土壤中的降解符合一级降解动力学方程C=0.0990e-0.0459t,半衰期为15.1d,r=-0.9826。在室内从紫外线、臭氧、pH值、原子辐照4个方面对氟虫腈消解状况进行了分析,发现紫外线照射25h时,氟虫腈降解率最高可达到90%;通入臭氧60min时,氟虫腈降解率最高可达48.5%;溶液pH为12时,氟虫腈在48h时降解率高达88.0%;而辐照处理对氟虫腈的降解效果不明显。

Application of ELISA in Degradation Analysis of Fipronil

Indirect competitive ELISA based on monoclonal antibody was applied to monitor the degradation kinetics of fipronil in vegetable and soil. Degradation kinetics of fipronil in vegetable followed pseudo-first-order kinetics equation C=0.192 7e~ -0.066 6t, and the half-life value was 10.4 d (r=-0.978 7); degradation kinetics of fipronil in soil followed pseudo-first-order kinetics equation C=0.099 0e~ -0.045 9t, and the half-life value was 15.1 d (r=-0.982 6). The immunological assay method was also used to evaluate the effect of UV-light irradiation, ozone supply in aqueous solution, pH value and Co-60 irradiation on the degradation of fipronil in the laboratory. When the sample was irradiated by UV-light for 25 h, supplied with ozone for 60 min, or treated with pH 12 solution for 48 h, the maximum dissipation rate of fipronil was 90%, 48.5% and 88.0% respectively, while Co-60 irradiation had little effect on the degradation of fipronil.