金荞麦叶保护酶与保护物质对增强UV-B辐射及干旱胁迫的响应

采用盆栽试验测定金荞麦在干旱和UV-B辐射胁迫下超氧化物歧化酶 （SOD） 活性、过氧化物酶 （POD）活性、总黄酮含量、游离脯氨酸和可溶性蛋白含量。试验结果显示在充足水分状况下，增强的UV|B辐射提高了金荞麦叶SOD活性、总黄酮含量、游离脯氨酸含量；增强的UV-B辐射在处理前期和后期提高了金荞麦叶POD活性；增强的UV|B辐射在处理前期增加了金荞麦叶可溶性蛋白含量。在干旱胁迫下，增强的UV-B辐射在处理中期提高了金荞麦叶SOD活性，而在处理前期和后期却降低了其SOD活性；增强的UV-B辐射在处理后期提高了金荞麦叶POD活性以及游离脯氨酸和可溶性蛋白含量。在干旱条件下，金荞麦通过酶系统和保护物质的调节减少胁迫带来的不利影响，增强试验设定强度的UV-B辐射在一定程度上有利于提高其对干旱的抗性。

Response of the protective enzymes and protective substances of Fagopyrum dibotrys under enhanced UV-B radiation and drought stress

This paper is about the influences of enhanced UV|B radiation in simulation and drought stress on the protective enzymes and protective substances of Fagopyrum dibotrys， and so as to provide a theoretical basis for the cultivation for F. dibotrys. We took experiments of potted plants， we mensurated the SOD activity， POD activity， total flavonoids contents， free proline contents and soluble protein contents of F. dibotrys under different drought stress and UV|B radiations. The results showed enhanced UV|B radiation can increase SOD activity， total flavonoids contents and free proline contents of F. dibotrys under adequate water condition. In the early and late stages of the treatment， POD activity of F. dibotrys was increased by enhanced UV-B radiation. In the early and late stages of the treatment， soluble protein contents of F. dibotrys was increased by enhanced UV-B radiation. In conditions of drought stress， enhanced UV-B radiation increased SOD activity of F. dibotrys in the middle stage of the treatment while decreased it in the early and late stages of the treatment. In the late stage of the treatment， enhanced UV-B radiation increased POD activity， free proline contents and soluble pro-n contents of F. dibotrys. In conditions of drought stress， F. dibotrys reduced the adverse effects by regulating protective enzyme system and protective substances， the enhanced UV-B radiation improved the resistance of F. dibotrys to drought stress to some extent.