水稻类病变突变体spl5胚性悬浮细胞系的构建

类病变突变体（lesion mimic mutant）是一类研究细胞死亡与抗病机制的有效工具。试验以水稻类病变突变体spl5为材料，构建了spl5突变体的胚性悬浮细胞系。结果表明诱导spl5突变体愈伤组织的最佳2，4-D浓度为2 mg·L-1，疏松的Ⅱ型胚性愈伤组织经悬浮继代培养3～4个月后可获得理想的胚性悬浮细胞系，FDA-PI染色结果表明该细胞悬浮体系活力高。水稻类病变突变体spl5胚性悬浮细胞系的建立可为进一步研究spl5突变体细胞死亡及其与病原菌互作的机制奠定基础。

Establishment of embryogenic suspension cell line of rice lesion mimic mutant spl5

Lesion mimic mutant is a powerful tool to study the mechanisms of cell death and disease resistance. This study established a suspension-cell line of spl5， a rice lesion mimic mutant. For spl5 mutant， the optimum concentration of 2，4-D was 2 mg·L-1 in callus induction and the ideal embryogenic suspension-cell line， from osteoporosis type Ⅱ embryogenic callus， was achieved after cultured 3-4 months in suspension medium. After FDA-PI staining， the suspension-cell line of spl5 mutant showed high viability， and could be used in the further study on the mechanism of cell death and interaction with pathogen in spl5 mutant.