甘蔗常用亲本遗传多样性和磷效率研究

本研究利用18对SSR分子标记对20份常用甘蔗亲本进行遗传多样性分析,实验结果表明,共扩增出410条带,每对引物的扩增带数为9～52条,平均23条;遗传相似性为0.522～0.693,平均为0.606,相似系数较低,亲本间遗传差异较大。SSR聚类分析结果表明:以相似系数0.61为标准,可以将20份常用甘蔗亲本分为四类,分类结果与系谱来源基本吻合,磷效率在四类甘蔗亲本材料间存在显著的类型差异。在亲本选配过程中,综合考虑亲本的SSR分子标记及磷效率评价结果,有利于提高亲本选择准确性和培育磷高效基因型。

Study on Genetic Diversity and P Efficiency of Commonly-used Sugarcane Parents

In this study,the genetic diversity among 20 commonly-used sugarcane genotypes with 18 pairs of SSR microsatellite markers as primers.A total of 410 alleles were obtained,and the number of alleles amplified by per marker ranged from 9 to 52 with an average of 23.The genetic similarity coefficients of the 20 sugarcane genotypes were from 0.522 to 0.693 with an average of 0.606,which revealed that the 20 sugarcane genotypes showed a relatively low level of genetic similarity while a relatively high level of g...