Effect of nutritional soil confected with Methane manure compared with ordinary nutritional soil (6 portion soil,3 portion manure and 1 portion sand)on cucumber (Jinchun No.4)seedling were studied.The results showed that there were significant difference in morphologic indexes,photosynthetic pigment,net photosynthesis rate,root vigor and activities of peroxidase (POD) and nitrate reductase(NR) among different nutritional soil,the optimal nutritional soil that confected with 7 portion soil 2 portion fen manure 1 portion sand.