| 马立克氏病肿瘤细胞表面抗原的提取与鉴定 |
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| 引用本文： | 穆杨,王玉东,张彦明等. 马立克氏病肿瘤细胞表面抗原的提取与鉴定[J]. 中国农学通报, 2005, 21(5): 48-48 |
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| 作者姓名： | 穆杨 王玉东 张彦明等 |
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| 作者单位： | 1. 西北农林科技大学物科技学院,陕西杨凌,712100
2. 农业部动物检疫所,山东青岛,266032 |
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| 基金项目： | 国家自然科学基金(30371067)，霍英东教育基金(91033)资助。 |
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| 摘 要： | 用MDVGA株感染1日龄SPF雏鸡,饲养至发病后获得MD肿瘤细胞,采用木瓜蛋白酶消化法从MD肿瘤细胞上提取马立克氏病肿瘤相关的表面抗原,经间接荧光抗体染色和间接ELISA鉴定,证明获得的粗提物中含有马立克氏病肿瘤相关的表面抗原,为进行马立克氏病肿瘤相关的表面抗原的进一步研究奠定了基础。
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| 关 键 词： | MD 木瓜蛋白酶 马立克氏病肿瘤相关的表面抗原 提取 鉴定 |
Separation and Identification of Marek's Disease Tumor-Associated Surface Antigen |
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| MU Yang,Wang Yudong,Zhang Yanming,Tong Dewen,Li Wei,LI Yuqing,Zheng Zengren. Separation and Identification of Marek's Disease Tumor-Associated Surface Antigen[J]. Chinese Agricultural Science Bulletin, 2005, 21(5): 48-48 |
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| Authors: | MU Yang Wang Yudong Zhang Yanming Tong Dewen Li Wei LI Yuqing Zheng Zengren |
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| Affiliation: | Mu Yang1,Wang Yudong2,Zhang Yanming1,Tong Dewen1,Li Wei2,Li Yuqing2,Zheng Zengren2 |
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| Abstract: | SPF chickens of one day were infected with Marek's disease virus GA and tumor cells were gained from diseased chickens. Marek's disease tumor-associated surface antigen was separated from MD tumor cell by using papain. It was proved that MATSA had been separated from tumor cell by indirect immunofluorescence assay and indirect enzyme linked immunosorbent assay. |
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| Keywords: | MD Papain Marek's disease tumor-associated surface antigen Separate Identification |
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