Attempts to eliminate <Emphasis Type="Italic">Candidatus</Emphasis> phytoplasma phoenicium from infected Lebanese almond varieties by tissue culture techniques combined or not with thermotherapy期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Attempts to eliminate Candidatus phytoplasma phoenicium from infected Lebanese almond varieties by tissue culture techniques combined or not with thermotherapy

Authors:	Lamis?Chalak author-information" > author-information__contact u-icon-before" > mailto:lchalak@lari.gov.lb" title=" lchalak@lari.gov.lb" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Ahmad?Elbitar,Reine?Rizk,Elia?Choueiri,Pascal?Salar,Joseph?M.?Bové

Affiliation:	(1) Department of Plant Biotechnology, Lebanese Agricultural Research Institute, Zahle, P.O. Box 287, Lebanon;(2) Department of Plant Pathology, Lebanese Agricultural Research Institute, Zahle, P.O. Box 287, Lebanon;(3) Institut National de la Recherche Agronomique, UMR GDPP, BP 81, 33 883 Villenave dOrnon cedex, France

Abstract:	Elimination of Candidatus phytoplasma phoenicium from two infected Lebanese varieties of almond by using different tissue culture techniques is reported. Except for the oxytetracycline therapy which totally inhibited the development of explants, stem cutting cultures associated with thermotherapy, shoot tip cultures associated or not with thermotherapy, and shoot tip micrografting were all suitable, either for shoot regeneration or for elimination of phytoplasma from the two varieties. However, stem cutting culture coupled with thermotherapy seemed to be the most effective for regeneration of phytoplasma-free plantlets.

Keywords:	almond chemotherapy in vitro micrografting nodal cuttings PCR test phytoplasma sanitation shoot tips thermotherapy
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