花生AhbZIP4基因克隆及其在侧枝发育中的表达分析

花生侧枝发育决定株型的建成,但有关花生侧枝发育的基因尚知之甚少。为探究AhbZIP4基因序列特征及在2种株型花生品种的组织器官中的表达模式,本研究从侧枝直立型花生‘冀花5号’（JH5）中克隆获得AhbZIP4基因（Arahy.CS824N.1）序列,并对其进行序列特征分析、亚细胞定位和不同株型品种组织器官表达分析。序列分析表明,该基因编码序列全长1 182 bp,编码393个氨基酸,预测蛋白质分子质量为42.10 kD,蛋白质二、三级结构具典型的bZIP拉链,无跨膜域和信号肽;系统进化分析发现,AhbZIP4与大豆GBF2和GBF2A同源性最高;亚细胞定位显示,AhbZIP4位于细胞核和细胞膜中;表达量分析说明,JH5和匍匐型花生M130除在结荚期时的花中表达差异不显著,在其他生育期的不同组织中均呈现显著或极显著表达差异。其中,2个品种在侧枝发育的15～25 d之间,AhbZIP4表达差异极显著,推测该基因可能间接影响花生株型的分化。研究结果将为进一步揭示花生侧枝发育及株型建成的分子机制提供理论基础。

Cloning of Ahb ZIP4 and its expression analysis in the lateral branches development in cultivated peanut (Arachis hypogaea L.) #br#

The development of the lateral branches determines the formation of plant type in cultivated peanut.However the genes related to the development of peanut lateral branches were poorly understood. In the present study, AhbZIP4(Arahy. CS824N.1) gene was cloned from variety 'Jihua 5'(JH5) with erect lateral branch, and its sequence characteristics, subcellular localization and tissue specific expression were analyzed. Firstly, the coding region of AhbZIP4 was 1 182 bp encoding 393 amino acids whose protein molecular weight is 42.10 kD. The protein contained a typical bZIP zipper, and there was no transmembrane domain and signal peptide. Secondly, phylogenetic analysis found that AhbZIP4 had the highest homology with GBF2 and GBF2A of soybean. Moreover, subcellular localization showed that AhbZIP4 was located in the cell nucleus and membrane. Finally, the expression analysis showed that AhbZIP4 expressed significantly or extremely significantly different in diverse tissues of JH5 and creeping peanut M130 in all the growth stages except for the flowers of the podding stage. AhbZIP4 expression was significantly different between the two varieties during the 15-25 days of lateral branch development. It is speculated that AhbZIP4 may affect peanut plant type indirectly. The results will provide a theoretical basis for further revealing the molecular mechanism of lateral branch development and plant type formation in peanut.